Chemoenzymatic design of heparan sulfate oligosaccharides

Authors
Liu, R.
Xu, Y.
Chen, M.
Weïwer, M.
Bridges, A.
DeAngelis, P.L.
Zhang, Q.
Linhardt, Robert J.
Liu, J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2010-10-29
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
Chemoenzymatic design of heparan sulfate oligosaccharides, R. Liu, Y. Xu, M. Chen, M. Weïwer, A. Bridges, P.L. DeAngelis, Q. Zhang, R. J. Linhardt, J. Liu, Journal of Biological Chemistry, 285, 34240-34249, 2010.
Abstract
Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C5-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.
Description
Journal of Biological Chemistry, 285, 34240-34249
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Biological Chemistry
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI
Open Access
A full text version is available in DSpace@RPI