Chemoenzymatic design of heparan sulfate oligosaccharides
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Authors
Liu, R.
Xu, Y.
Chen, M.
Weïwer, M.
Bridges, A.
DeAngelis, P.L.
Zhang, Q.
Linhardt, Robert J.
Liu, J.
Issue Date
2010-10-29
Type
Article
Language
en_US
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C5-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.
Description
Journal of Biological Chemistry, 285, 34240-34249
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Chemoenzymatic design of heparan sulfate oligosaccharides, R. Liu, Y. Xu, M. Chen, M. Weïwer, A. Bridges, P.L. DeAngelis, Q. Zhang, R. J. Linhardt, J. Liu, Journal of Biological Chemistry, 285, 34240-34249, 2010.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
1083351X
219258
219258