Chemoenzymatic design of heparan sulfate oligosaccharides

Liu, R.
Xu, Y.
Chen, M.
Weïwer, M.
Bridges, A.
DeAngelis, P.L.
Zhang, Q.
Linhardt, Robert J.
Liu, J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Chemoenzymatic design of heparan sulfate oligosaccharides, R. Liu, Y. Xu, M. Chen, M. Weïwer, A. Bridges, P.L. DeAngelis, Q. Zhang, R. J. Linhardt, J. Liu, Journal of Biological Chemistry, 285, 34240-34249, 2010.
Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C5-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.
Journal of Biological Chemistry, 285, 34240-34249
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Biological Chemistry
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