A novel laminin-binding protein mediates microbial-endothelial cell interactions and facilitates dissemination of Lyme disease pathogens

Bista, Sandhya
Singh, Preeti
Bernard, Quentin
Yang, Xiuli
Hart, Thomas
Lin, Yi Pin
Kitsou, Chrysoula
Singh Rana, Vipin
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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A novel laminin-binding protein mediates microbial-endothelial cell interactions and facilitates dissemination of Lyme disease pathogens, S. Bista, P. Singh, Q. Bernard, X. Yang, T. Hart, Y.-P. Lin, C. Kitsou, V. S. Rana, F. Zhang, R. J. Linhardt, K. Zhang, D. R. Akins, L. Hritzo, Y. Kim, D. J. Grab, J. S. Dumler, U. Pal, Journal of Infectious Diseases, 221, 1438–1447, 2020.
Borrelia burgdorferi conserved gene products BB0406 and BB0405, members of a common B. burgdorferi paralogous gene family, share 59% similarity. Although both gene products can function as potential porins, only BB0405 is essential for infection. Here we show that, despite sequence homology and coexpression from the same operon, both proteins differ in their membrane localization attributes, antibody accessibility, and immunogenicity in mice. BB0406 is required for spirochete survival in mammalian hosts, particularly for the disseminated infection in distant organs. We identified that BB0406 interacts with laminin, one of the major constituents of the vascular basement membrane, and facilitates spirochete transmigration across host endothelial cell barriers. A better understanding of how B. burgdorferi transmigrates through dermal and tissue vascular barriers and establishes disseminated infections will contribute to the development of novel therapeutics to combat early infection.
Journal of Infectious Diseases, 221, 1438–1447
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Infectious Diseases