Improved Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Detection of Glycosaminoglycan Disaccharides as Cesium Salts

Authors
Laremore, Tatiana N.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2007-04-04
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Full Citation
Improved Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Detection of Glycosaminoglycan Disaccharides as Cesium Salts, T.N. Laremore, R.J. Linhardt, Rapid Communications in Mass Spectrometry, 21, 1315-1320, 2007.
Abstract
A new method is presented for the identification of oligosaccharides obtained by enzymatic digestion of hyaluronan (HA) with bacterial hyaluronidase (E.C. 4.2.2.1, from Streptomyces hyalurolyticus) using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOFMS). Mixtures containing HA oligosaccharides of tetrasaccharide (4-mer)–34-mer were analyzed using this method. The carboxyl groups of the glucuronate residues in the prepared HA oligomers, were modified as the acidic form (—COOH), sodium salts (—COONa), organic ammonium salts, or methylesters before MALDI-TOFMS measurement. Among these samples, the methylester form of glucuronate residues in HA oligosaccharides, prepared by methylation using trimethylsilyl diazomethane, afforded high sensitivity for spectra. This simple modification method for carboxyl group methylation of acidic polysaccharides [Hirano et al., Carbohydr. Res., 340, (2005) 2297–2304] provides samples suitable for MALDI-TOF mass spectrometric analysis throughout a significantly enhanced range of masses.
Description
Rapid Communications in Mass Spectrometry, 21, 1315-1320
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Wiley
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Rapid Communications in Mass Spectrometry
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI