Capillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase
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Authors
Lee, K.B.
Kim, Y.S.
Linhardt, Robert J.
Issue Date
1991-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Capillary zone electrophoresis with fluorescence detection was used to analyze the products formed by chitinase acting on N-acetylchitooligosaccharide-fluorescent conjugates. Six oligosaccharides of the structure [N-acetylglucosamine(1----4)]n (where n = 1-6) were conjugated to 7-amino-1,3-naphthalene disulfonic acid by reductive amination. Each oligosaccharide-fluorescent conjugate was purified by preparative gradient polyacrylamide gel electrophoresis, semi-dry electrotransfer to a positively-charged nylon membrane and recovered by washing the membrane with salt solution. The products formed by treating each oligosaccharide-fluorescent conjugate with chitinase were analyzed by capillary zone electrophoresis. The chitinase treatment hexasaccharide-fluorescent conjugate was also examined kinetically to study the action pattern of this enzyme.
Description
Electrophoresis, 12, 636-640
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Capillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase, K.B. Lee, Y.S. Kim, R.J. Linhardt, Electrophoresis, 12, 636-640 (1991).
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15222683
1730835
1730835