Domain structure elucidation of human decorin glycosaminoglycans
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Authors
Laremore, Tatiana N.
Ly, Mellisa
Zhang, Zhenqing
Solakyildirim, Kemal
McCallum, Scott A.
Owens, Richard T.
Linhardt, Robert J.
Issue Date
2010-10-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The structure of the GAG (glycosaminoglycan) chain of recombinantly expressed decorin proteoglycan was examined using a combination of intact-chain analysis and domain compositional analysis. The GAG had a number-average molecular mass of 22 kDa as determined by PAGE. NMR spectroscopic analysis using two-dimensional correlation spectroscopy indicated that the ratio of glucuronic acid to iduronic acid in decorin peptidoglycan was 5 to 1. GAG domains terminated with a specific disaccharide obtained by enzymatic degradation of decorin GAG with highly specific endolytic and exolytic lyases were analysed by PAGE and further depolymerized with the enzymes. The disaccharide compositional profiles of the resulting domains were obtained using LC with mass spectrometric and photometric detection and compared with that of the polysaccharide. The information obtained through the disaccharide compositional profiling was combined with the NMR and PAGE data to construct a map of the decorin GAG sequence motifs.
Description
Biochemical Journal 431, 199-205
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Domain structure elucidation of human decorin glycosaminoglycans, T. N. Laremore, M. Ly, Z. Zhang, K. Solakyildirim, S. McCallum, R. T. Owens, R. J. Linhardt, Biochemical Journal 431, 199-205, 2010.
Publisher
Portland Press
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
14708728
2646021
2646021