Signal Amplification of Target Protein on Heparin Glycan Microarray
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Authors
Park, Tae Joon
Lee, Moo Yeal
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2008-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A heparin glycan chip (HepGlyChip) with a 4800-fold enhanced signal-to-noise ratio as compared with the control without heparin was developed for high-throughput analysis of heparin-protein interactions for new drug development and for screening biological samples in diagnostic applications. As a proof of concept, a heparin glycan microarray was prepared on a poly(styrene-co-maleic anhydride) (PS-MA)-coated glass slide. Heparin was covalently immobilized on poly-l-lysine (PLL) layer with multiple binding sites by sulfo-ethylene glycol bis(succinimidylsuccinate) (sulfo-EGS), increasing the signal-to-noise ratio, minimizing nonspecific binding of target proteins, and resulting in a three-dimensional (3D) structure on the HepGlyChip. This on-chip signal amplification platform was successfully demonstrated by probing the heparin microarray with the highly specific heparin-binding protein antithrombin III (AT III).
Description
Analytical Biochemistry, 383, 116–121
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Signal Amplification of Target Protein on Heparin Glycan Microarray, T.-J. Park, M.-Y. Lee, J. S. Dordick, R. J. Linhardt, Analytical Biochemistry, 383, 116–121, 2008.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697