Comprehensive analysis of chondroitin sulfate and aggrecan in the head cartilage of bony fishes: Identification of proteoglycans in the head cartilage of sturgeon

Authors
Shionoya, Kento
Suzuki, Takehiro
Takada, Mako
Sato, Kazuki
Onishi, Shoichi
Dohmae, Naoshi
Nishino, Koichiro
Wada, Takeshi
Linhardt, Robert J.
Toida, Toshihiko
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2022-05-31
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Comprehensive analysis of chondroitin sulfate and aggrecan in the head cartilage of bony fishes: Identification of proteoglycans in the head cartilage of sturgeon, K. Shionoya, T. Suzuki, M. Takada, K. Sato, S. Onishi, N. Dohmae, K. Nishino, T. Wada, R. J. Linhardt, T. Toida, K. Higashi, International Journal of Biological Macromolecules, 208, 333–342, 2022.
Abstract
Cartilage in the head of sturgeon or salmon has been gaining attention as a rich source of functional chondroitin sulfate (CS) or proteoglycans. Although the cartilage was found in the heads of other bony fishes, the structure of CS and its core protein, especially aggrecan, was not fully investigated. In this study, comprehensive analysis of CS and aggrecan in the head cartilage of 10 bony fishes including sturgeon and salmon was performed. The 4-O-sulfation to 6-O-sulfation ratio (4S/6S ratio; S: sulfate residue) of CS in Perciformes was ≧1.0, while the 4S/6S ratios of CS from sturgeons and salmon were less than 0.5. Dot blotting and proteomic analysis revealed that aggrecan was a major core protein in head cartilage of all bony fishes. These results suggest that the head cartilage of bony fishes is a promising source for the preparation of CS or proteoglycans as a health food ingredient.
Description
International Journal of Biological Macromolecules, 208, 333–342
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
International Journal of Biological Macromolecules
https://harc.rpi.edu/
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