Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
Authors
Cai, Chao
Solakyildirim, Kemal
Yang, Bo
Beaudet, Julie M.
Weyers, Amanda
Linhardt, Robert J.
Zhang, Fuming
Issue Date
2012-01-04
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Chondroitin sulfate-E (chondroitin-4, 6-disulfate) was prepared from chondroitin sulfate-A (chondroitin-4 - sulfate) by regioselective sulfonation, performed using trimethylamine sulfur trioxide in formamide under argon. The structure of semi-synthetic chondroitin sulfate-E was analyzed by PAGE, 1H NMR, 13C NMR, 2D NMR and disaccharide analysis and compared with natural chondroitin sulfate-E. Both semi-synthetic and natural chondroitin sulfate-E were each biotinylated and immobilized on BIAcore SA biochips and their interactions with fibroblast growth factors displayed very similar binding kinetics and binding affinities. The current semi-synthesis offers an economical approach for the preparation of the rare chondroitin sulfate-E from the readily available chondroitin sulfate-A.
Description
Carbohydrate Polymers, 87, 822-829
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A, C. Cai, K. Solakyildirim, B. Yang, J. M. Beaudet, A. Weyers, R. J. Linhardt, F. Zhang, Carbohydrate Polymers, 87, 822-829, 2012.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
1448617