Mapping and Sequencing of Oligosaccharides by Electrophoresis

Linhardt, Robert J.
Liu, J.
Han, X.-J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Mapping and Sequencing of Oligosaccharides by Electrophoresis R.J. Linhardt, J. Liu, X. -J. Han, Trends in Glycoscience and Glycotechnology, 5, 181-192 (1993).
The analysis of oligosaccharides and polysaccharides takes on increased importance with the discovery of each new biological activity attributable to this important class of biopolymers. Our understanding of other biopolymers, pro-teins and nucleic acids, has relied heavily on electrophoresis. This minireview describes the application of capillary electro-phoresis and gradient polyacrylamide gel electrophoresis to saccharide compositional analysis, oligosaccharide mapping and oligosaccharide and polysaccharide sequencing. These ap-proaches are applicable to both acidic polysaccharides (i.e., glycosaminoglycans) and neutral, glycoprotein-derived oligo-saccharides. Separation by electrophoresis can be driven by the inherent charge of a carbohydrate, through the complexation of a carbohydrate with a charged molecule, or through derivati-zation reactions to prepare charged carbohydrate conjugates. New approaches including fluorescence labeling provide the high detection sensitivity required for microanalysis. The high resolution necessary for oligosaccharide analysis can be achieved using capillary electrophoresis or two-dimensional gel electrophoresis. Preparative gradient polyacrylamide gel electrophoresis can also be used to obtain pure oligosaccharides for structural studies. Future prospects for the complete se-quence analysis of oligosaccharides and polysaccharides by electrophoresis are discussed.
Trends in Glycoscience and Glycotechnology, 5, 181-192
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Trends in Glycoscience and Glycotechnology