Mapping and Sequencing of Oligosaccharides by Electrophoresis

Authors
Linhardt, Robert J.
Liu, J.
Han, X.-J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1993-02-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Mapping and Sequencing of Oligosaccharides by Electrophoresis R.J. Linhardt, J. Liu, X. -J. Han, Trends in Glycoscience and Glycotechnology, 5, 181-192 (1993).
Abstract
The analysis of oligosaccharides and polysaccharides takes on increased importance with the discovery of each new biological activity attributable to this important class of biopolymers. Our understanding of other biopolymers, pro-teins and nucleic acids, has relied heavily on electrophoresis. This minireview describes the application of capillary electro-phoresis and gradient polyacrylamide gel electrophoresis to saccharide compositional analysis, oligosaccharide mapping and oligosaccharide and polysaccharide sequencing. These ap-proaches are applicable to both acidic polysaccharides (i.e., glycosaminoglycans) and neutral, glycoprotein-derived oligo-saccharides. Separation by electrophoresis can be driven by the inherent charge of a carbohydrate, through the complexation of a carbohydrate with a charged molecule, or through derivati-zation reactions to prepare charged carbohydrate conjugates. New approaches including fluorescence labeling provide the high detection sensitivity required for microanalysis. The high resolution necessary for oligosaccharide analysis can be achieved using capillary electrophoresis or two-dimensional gel electrophoresis. Preparative gradient polyacrylamide gel electrophoresis can also be used to obtain pure oligosaccharides for structural studies. Future prospects for the complete se-quence analysis of oligosaccharides and polysaccharides by electrophoresis are discussed.
オリコ糖や多糖の分析は、オリコ糖や多糖に起因する生物活性の発見とともにますますその重要性が認識されつつある。タンパク質や核酸など、その他の生体高分子は電気泳動を利用する分析法に大きく依存レている。本論文ではキャピラリー電気泳動およびグラジエントポリアクリルアミドゲル電気泳動を用いる構成糖分析、オリゴ糖マッピング、オリコ糖および多糖のシークエンス分析について紹介する。今回紹介する方法は酸性多糖(グリコサミノグリカン)や中性の糖タンパク質に由来するオリコ聴に適用できる。糖質を電気泳動により分離する手段として、糖が本来持っている電荷、糖とキャリアー緩衝液中の荷電分子との錯体形成、および誘導体化により糖質に電荷を導入するという3種類の方法を利用することができる。蛍光標識などの最近開発された方法は、ミクロ分析が必要とされる高感度分析を可能にする。オリコ糖の分析に必要とされる高い分離能はキャピラリー電気泳動や二次元ゲル電気泳動により達成される。分取グラジエントゲル電気泳動は構造解析用のオリコ糖を純粋に得るために有用な手段となる。電気泳動を用いるオリゴ糖や多糖の完全シークエンス分析の将来についても述べる。
Description
Trends in Glycoscience and Glycotechnology, 5, 181-192
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Trends in Glycoscience and Glycotechnology
https://harc.rpi.edu/
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