CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

Authors
Cress, Brady F.
Leitz, Quentin D.
Kim, Daniel C.
Amore, Teresita D.
Suzuki, Jon Y.
Linhardt, Robert J.
Koffas, Mattheos A.G.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2017-01-17
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production, B. F. Cress, Q. D. Leitz, D. C. Kim, T. D. Amore, J. Y. Suzuki, R. J. Linhardt, Mattheos A. G. Koffas, Microbial Cell Factories, 16, (10), 1-15, 2017.
Abstract
Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit pH-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. In this work, we report for the first time the extension of the reconstituted plant anthocyanin pathway from (+)-catechin to O-methylated anthocyanins in a microbial production system, an effort which requires simultaneous co-option of the endogenous metabolites UDP-glucose and S-adenosyl-L-methionine (SAM or AdoMet).
Description
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Springer Nature
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Microbial Cell Factories
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI
CC BY — Creative Commons Attribution