CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
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Authors
Cress, Brady F.
Leitz, Quentin D.
Kim, Daniel C.
Amore, Teresita D.
Suzuki, Jon Y.
Linhardt, Robert J.
Koffas, Mattheos A.G.
Issue Date
2017-01-17
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit pH-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. In this work, we report for the first time the extension of the reconstituted plant anthocyanin pathway from (+)-catechin to O-methylated anthocyanins in a microbial production system, an effort which requires simultaneous co-option of the endogenous metabolites UDP-glucose and S-adenosyl-L-methionine (SAM or AdoMet).
Description
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
Full Citation
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production, B. F. Cress, Q. D. Leitz, D. C. Kim, T. D. Amore, J. Y. Suzuki, R. J. Linhardt, Mattheos A. G. Koffas, Microbial Cell Factories, 16, (10), 1-15, 2017.
Publisher
Springer Nature
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
14752859