Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers

Authors
Wang, Q.
Linhardt, Robert J.
Dordick, J.S.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1999
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers, Q. Wang, R.J. Linhardt, J.S. Dordick BiotechnologyTechniques, 13, 463-467, 1999.
Abstract
A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.
Description
BiotechnologyTechniques, 13, 463-467
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
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