Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers
No Thumbnail Available
Authors
Wang, Q.
Linhardt, Robert J.
Dordick, J.S.
Issue Date
1999
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.
Description
BiotechnologyTechniques, 13, 463-467
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers, Q. Wang, R.J. Linhardt, J.S. Dordick BiotechnologyTechniques, 13, 463-467, 1999.