Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers

Wang, Q.
Linhardt, Robert J.
Dordick, J.S.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Affinity Chromatography Using Enzymatically Synthesized Nucleotide-ContainingDNA Binding Polymers, Q. Wang, R.J. Linhardt, J.S. Dordick BiotechnologyTechniques, 13, 463-467, 1999.
A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.
BiotechnologyTechniques, 13, 463-467
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