Signal amplification by Glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology
Loading...
Authors
Kwon, Seok Joon
Lee, Kyung Bok
Solakyildirim, Kemal
Masuko, Sayaka
Ly, Mellisa
Zhang, Fuming
Li, Lingyun
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2012-11-19
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.
Description
Angewandte Chemie, 51, 11800 –11804
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Signal amplification by Glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology, S.-J. Kwon, K.-B. Lee, K. Solakyildirim, S. Masuko, M. Ly, F. Zhang, L. Li, J. S. Dordick, R. J. Linhardt, Angewandte Chemie, 51, 11800 –11804, 2012.
Publisher
Wiley
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15213773
14337851
14337851