Signal amplification by Glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology

Authors
Kwon, Seok Joon
Lee, Kyung Bok
Solakyildirim, Kemal
Masuko, Sayaka
Ly, Mellisa
Zhang, Fuming
Li, Lingyun
Dordick, Jonathan S.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
Loading...
Thumbnail Image
Other Contributors
Issue Date
2012-11-19
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
Full Citation
Signal amplification by Glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology, S.-J. Kwon, K.-B. Lee, K. Solakyildirim, S. Masuko, M. Ly, F. Zhang, L. Li, J. S. Dordick, R. J. Linhardt, Angewandte Chemie, 51, 11800 –11804, 2012.
Abstract
Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.
Description
Angewandte Chemie, 51, 11800 –11804
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Wiley
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Angewandte Chemie - International Edition
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI