Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM
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Authors
Yu, Yanlei
Zhang, Fuming
Colón, Wilfredo
Linhardt, Robert J.
Xia, Ke
Issue Date
2019-02-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring. CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan. The amounts and disaccharide composition of these GAGs differed from those found in human plasma. This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
Description
Analytical Biochemistry,567, 82–84
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM, Y. Yu, F. Zhang, W. Colón, R. J. Linhardt, Ke Xia, Analytical Biochemistry,567, 82–84, 2019.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697