Structural characterization of pharmaceutical heparins prepared from different animal tissues
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Authors
Fu, Li
Li, Guoyun
Yang, Bo
Onishi, Akihiro
Li, Lingyun
Sun, Peilong
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2013-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Although most pharmaceutical heparin used today is obtained from porcine intestine, heparin has historically been prepared from bovine lung and ovine intestine. There is some regulatory concern about establishing the species origin of heparin. This concern began with the outbreak of mad cow disease in the 1990s and was exacerbated during the heparin shortage in the 2000s and the heparin contamination crisis of 2007-2008. Three heparins from porcine, ovine, and bovine were characterized through state-of-the-art carbohydrate analysis methods with a view profiling their physicochemical properties. Differences in molecular weight, monosaccharide and disaccharide composition, oligosaccharide sequence, and antithrombin III-binding affinity were observed. These data provide some insight into the variability of heparins obtained from these three species and suggest some analytical approaches that may be useful in confirming the species origin of a heparin active pharmaceutical ingredient.
Description
Journal of Pharmaceutical Science, 102, 1447-1457
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Structural characterization of pharmaceutical heparins prepared from different animal tissues, L. Fu, G. Li, B. Yang, A. Onishi, L. Li, P. Sun, F. Zhang, R.J. Linhardt, Journal of Pharmaceutical Science, 102, 1447-1457, 2013.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15206017
223549
223549