Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix
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Authors
Park, Youmie
Zhang, Zhenqing
Linhardt, Robert J.
LeMosy, Ellen K.
Issue Date
2008-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible for generating this spatial information during oogenesis through sulfation of unknown glycans. Although Pipe has sequence homology to glycosaminoglycan 2-O-sulfotransferases, its activity and authentic substrates have not been demonstrated and genetic evidence has argued against a role for glycosaminoglycans in dorsoventral polarity establishment. Here, direct examination of matrix glycosaminoglycans demonstrates that pipe-mutant matrix shows decreased tri-sulfated heparan sulfate compared to wild-type matrix, with correspondingly increased 2-O-sulfated heparan sulfate. Chondroitin sulfate was not detected in this matrix. These results suggest that Pipe promotes 6-O- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-O-sulfation. We discuss the possible significance of these unexpected findings and how they might be reconciled with the genetic data.
Description
Fly, 2, 1-5
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix, Y. Park, Z. Zhang, R.J. Linhardt, E. K. LeMosy, Fly, 2, 1-5, 2008.
Publisher
Taylor and Francis
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
19336942
19336934
19336934