Fundamental and applied studies of polymer membranes

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Authors
Imbrogno, Joseph
Issue Date
2016-05
Type
Electronic thesis
Thesis
Language
ENG
Keywords
Chemical and biological engineering
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Abstract
Stem cells are currently grown on biologically based substrates, which do not allow for optimization or tailoring to specific cell type. Using our high throughput screening platform, we were able to determine that (N-[3-(dimethylamino)propyl] methacrylamide), DMAPMA, supported strong attachment and long-term self-renewal of mouse embryonic stem (ES) cells while preventing differentiation (maintaining pluripotency). After developing this platform, it was used to screen for a surface that could instead induce differentiation of bovine and human retinal pigment epithelium (RPE) cells while promoting cell growth. Several PEG based surfaces were able to induce cobblestone morphology of the RPE cells, which is indicative of differentiation.
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May 2016
School of Engineering
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Rensselaer Polytechnic Institute, Troy, NY
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