Detection of Glycosaminoglycans as a Copper (II) Complex in Capillary Electrophoresis

Toida, T.E.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Detection of Glycosaminoglycans as a Copper (II) Complex in Capillary Electrophoresis, T. E. Toida, R.J. Linhardt, Electrophoresis, 17, 341-346, 1996.
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronic acid were analyzed by reversed polarity capillary electrophoresis. Detection was achieved at 240 nm based on the formation of a copper (II) complex in copper sulfate solution at low pH. Glycosaminoglycans having a high ratio of iduronic acid to glucuronic acid, as well as ones having lower molecular weight, gave the highest detection sensitivity using this method. Detection of the copper (II)-heparin complex is extremely sensitive, permitting the analysis of as little as 10−9 g. This method was also successfully applied to the analysis of heparin oligosaccharides that lacked a chromophore prepared from heparin using controlled, low pH nitrous acid depolymerization.
Electrophoresis, 17, 341-346
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Rensselaer Polytechnic Institute, Troy, NY