Variation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica)

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Authors
Park, Y.
Zhang, Z.
Laremore, T.N.
Li, B.
Sim, J.S.
Im, A.R.
Ahn, M.Y.
Kim, Y.S.
Linhardt, Robert J.
Issue Date
2008
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha-L-IdoAp2S(1-->, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex(2-4)-HexNAc(2)), high mannose (Hex(5-9)-HexNAc(2)), and complex (Hex(3)-HexNAc(2-10)) types. None showed core fucosylation.
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Glycoconjugate Journal, 25, 863-877
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Full Citation
Variation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica). Y. Park, Z. Zhang, T. N. Laremore, B. Li, J.-S. Sim, A.-R. Im, M. Y. Ahn, Y. S. Kim, R.J. Linhardt Glycoconjugate Journal, 25, 863-877, 2008.
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Springer
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