Increase in the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells by an O-Hexanoyl Low Molecular Weight Heparin Derivative
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Authors
Garg, Hari G.
Hales, Charles A.
Yu, Lunyin
Butler, Melissa
Islam, Tasneem
Xie, Jin
Linhardt, Robert J.
Issue Date
2006-11-06
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Proliferation of pulmonary artery smooth muscle cells (PASMCs) appears to play a significant role in chronic pulmonary hypertension. The proliferation of PASMCs is strongly inhibited by some commercial heparin preparations. Heparin fragments were prepared by periodate treatment, followed by sodium borohydride reduction, to enhance potency. The tributylammonium salt of this fragmented heparin was O-acylated with hexanoic anhydride. Gradient polyacrylamide gel electrophoresis showed that the major heparin fragment contained eight disaccharide units. NMR analysis showed that approximately one hexanoyl group per disaccharide residue was present. The O-hexanoyl heparin fragments were assayed for growth inhibitory effect on bovine PASMCs in culture. This derivative was found to be more effective in growth inhibition of bovine PASMCs in culture than the heparin from which it was derived. In the future, it is envisioned that this or similar derivatives may be an effective treatment for pulmonary hypertension.
Description
Carbohydrate Research, 341, 2607–2612
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Increase in the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells by an O-Hexanoyl Low Molecular Weight Heparin Derivative, H. G. Garg, N. Cindhuchao, C. A. Hales, M. Butler, T. Islam, R. J. Linhardt, Carbohydrate Research, 341, 2607–2612, 2006.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
86215