Click-coated, heparinized, decellularized vascular grafts

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Authors
Dimitrievska, Sashka
Cai, Chao
Weyers, Amanda
Balestrini, Jenna L.
Lin, Tylee
Sundaram, Sumati
Hatachi, Go
Spiegel, David A.
Kyriakides, Themis R.
Miao, Jianjun
Issue Date
2015-02-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
A novel method enabling the engineering of a dense and appropriately oriented heparin-containing layer on decellularized aortas has been developed. Amino groups of decellularized aortas were first modified to azido groups using 3-azidobenzoic acid. Azide-clickable dendrons were attached onto the azido groups through "alkyne-azide" click chemistry, affording a tenfold amplification of adhesions sites. Dendron end groups were finally decorated with end-on modified heparin chains. Heparin chains were oriented like heparan sulfate groups on native endothelial cells surface. X-ray photoelectron spectroscopy, nuclear magnetic resonance imaging, mass spectrometry and Fourier transform infrared FTIR spectroscopy were used to characterize the synthesis steps, building the final heparin layered coatings. The continuity of the heparin coating was verified using fluorescent microscopy and histological analysis. The efficacy of heparin linkage was demonstrated with factor Xa anti-thrombogenic assay and platelet adhesion studies. The results suggest that oriented heparin immobilization to decellularized aortas may improve the in vivo blood compatibility of decellularized aortas and vessels.
Description
Acta Biomateriala, 13, 177–187
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Full Citation
Click-coated, heparinized, decellularized vascular grafts, S. Dimitrievska, C. Cai, A. Weyers, T. Lin, S. Sundaram, D. A. Spiegel, T. R. Kyriakides, J. Miao, G. Li, L. Niklason, R. J. Linhardt, Acta Biomateriala, 13, 177–187, 2015.
Publisher
Elsevier
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ISSN
18787568
17427061
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