Structure Elucidation of a Novel Acidic Tetrasaccharide and Hexasaccharide Derived From a Chemically Modified Heparin

Desai, Umesh R.
Wang, Hui Ming
Kelly, Therese R.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Nature of the Interaction of Heparin with Acidic Fibroblast Growth Factor, H. Mach, D.B. Volkin, C.J. Burke, C.R. Middaugh, R.J. Linhardt, J. Fromm, D. Loganathan, L. Mattsson, Biochemistry, 32, 5480-5489 (1993).
In the course of development of potentially more bioactive forms of heparin for use as an anticoagulant, we had occasion to prepare a modified heparin first reported by Jaseja et al.‘. Treatment of unfractionated bovine lung heparin with an alkaline solution at high temperature (105-110°C) for 24 h, according to method developed by Rej and Perlin3, results in a modified heparin. To structurally characterize the sample of modified heparin, it was depolymerized with heparinase (heparin lyase I, EC This resulted in the isolation of the hitherto unreported tetrasaccharide and hexasaccharide. This note describes the isolation and structure elucidation of two novel oligosaccharides. The solution conformation of these oligosaccharides may aid in developing a better understanding of the conformational preferences of the uranic acid residues.
Carbohydrate Research, 241, 249-259
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Research