Tip-enhanced Raman imaging of single-stranded DNA with single base resolution
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Authors
He, Zhe
Han, Zehua
Kizer, Megan
Linhardt, Robert J.
Wang, Xing
Sinyukov, Alexander M.
Wang, Jizhou
Deckert, Volker
Sokolov, Alexei V.
Hu, Jonathan
Issue Date
2019-01-16
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Tip-enhanced Raman scattering (TERS) is a promising optical and analytical technique for chemical imaging and sensing at single molecule resolution. In particular, TERS signals generated by a gap-mode configuration where a silver tip is coupled with a gold substrate can resolve a single-stranded DNA (ssDNA) molecule with a spatial resolution below 1 nm. To demonstrate the proof of subnanometer resolution, we show direct nucleic acid sequencing using TERS of a phage ssDNA (M13mp18). M13mp18 provides a known sequence and, through our deposition strategy, can be stretched (uncoiled) and attached to the substrate by its phosphate groups, while exposing its nucleobases to the tip. After deposition, we scan the silver tip along the ssDNA and collect TERS signals with a step of 0.5 nm, comparable to the bond length between two adjacent DNA bases. By demonstrating the real-time profiling of a ssDNA configuration and furthermore, with unique TERS signals of monomeric units of other biopolymers, we anticipate that this technique can be extended to the high-resolution imaging of various nanostructures as well as the direct sequencing of other important biopolymers including RNA, polysaccharides, and polypeptides.
Description
Journal of the American Chemical Society, 141, 753−757
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Tip-enhanced Raman imaging of single-stranded DNA with single base resolution, Z. He, Z. Han, M. Kizer, R. Linhardt, X. Wang, A. Sinyukov, J. Wang, V. Deckert, A. Sokolov, J. Hu, M. Scully, Journal of the American Chemical Society, 141, 753−757, 2019.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15205126
27863
27863