Conjugation of ginsenoside Rg3 with gold nanoparticles

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Authors
Park, Youmie
Im, A. Rang
Joo, Eun Ji
Lee, Jihye
Park, Hyeung Geun
Kang, Young Hwa
Linhardt, Robert J.
Kim, Yeong Shik
Issue Date
2011-01-20
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Ginsenoside Rg3 was reported to have important biological activities. We demonstrate conjugation and quantification procedures of ginsenoside Rg3 to gold nanoparticles for future biological and medical applications. Ginsenoside Rg3 was conjugated to spherical gold nanoparticles using a bifunctional heptaethylene glycol linker. The sulfhydryl group of heptaethylene glycol was adsorbed onto gold nanoparticles, and carboxylic acid end of heptaethylene glycol was bonded through a hydroxyl group of Rg3 via ester bond formation. The conjugation of Rg3 was characterized with various spectroscopic techniques, high resolution-transmission electron microscopy, and using Rg3 monoclonal antibody. These results suggest that ginsenoside Rg3 is successfully conjugated to gold nanoparticles via heptaethylene glycol linker. The quantification was performed by using Rg3 monoclonal antibody without interference of gold's intrinsic color.
Description
Bulletin of the Korean Chemical Society, 32, 286-290
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Full Citation
Conjugation of ginsenoside Rg3 with gold nanoparticles, Y. Park, A.-R. Im, E. J. Joo, J. Lee, H.-G. Park, R. J. Linhardt, Y. S. Kim, Bulletin of the Korean Chemical Society, 32, 286-290, 2011.
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ISSN
12295949
2532964
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