Factors released from endothelial cells exposed to flow impact adhesion, proliferation, and fate choice in the adult NSC lineage
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Authors
Dumont, C.M.
Piselli, J.
Kazi, N.
Bowman, E.
Li, G.
Linhardt, Robert J.
Temple, S.
Dai, G.
Thompson, D.M.
Issue Date
2017
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The microvasculature within the neural stem cell (NSC) niche promotes self-renewal and regulates lineage progression. Previous work identified endothelial-produced soluble factors as key regulators of neural progenitor cell (NPC) fate and proliferation; however, endothelial cells (ECs) are sensitive to local hemodynamics, and the effect of this key physiological process has not been defined. In this study, we evaluated adult mouse NPC response to soluble factors isolated from static or dynamic (flow) EC cultures. Endothelial factors generated under dynamic conditions significantly increased neuronal differentiation, while those released under static conditions stimulated oligodendrocyte differentiation. Flow increases EC release of neurogenic factors and of heparin sulfate glycosaminoglycans that increase their bioactivity, likely underlying the enhanced neuronal differentiation. Additionally, endothelial factors, especially from static conditions, promoted adherent growth. Together, our data suggest that blood flow may impact proliferation, adhesion, and the neuron-glial fate choice of adult NPCs, with implications for diseases and aging that reduce flow.
Description
Stem Cells and Development, 26, 1199-1213
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Factors released from endothelial cells exposed to flow impact adhesion, proliferation, and fate choice in the adult NSC lineage, C. M. Dumont, J. Piselli, N. Kazi, E. Bowman, G. Li, R. J. Linhardt, S. Temple, G. Dai, D. M. Thompson, Stem Cells and Development, 26, 1199-1213, 2017.