Structure-activity relationship of citrus segment membrane RG-I pectin against galectin-3: The galactan is not the only important factor
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Authors
Wu, Dongmei
Zheng, Jiaqi
Hu, Weiwei
Zheng, Xiaoliang
He, Qiaojun
Linhardt, Robert J.
Ye, Xingqian
Chen, Shiguo
Issue Date
2020-10-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Rhamnogalacturonan I (RG-I) pectin are regarded as strong galectin-3 (Gal-3) antagonist because of galactan sidechains. The present study focused on discussing the effects of more structural regions in pectin on the anti-Gal-3 activity. The water-soluble pectin (WSP) recovered from citrus canning processing water was categorized as RG-I pectin. The controlled enzymatic hydrolysis was employed to sequentially remove the α-1,5-arabinan, homogalaturonan and β-1,4-galactan in WSP. The Gal-3-binding affinity KD (kd/ka) of WSP and debranched pectins were calculated to be 0.32 μM, 0.48 μM, 0.56 μM and 1.93 μM. Moreover, based on the more sensitive cell line (MCF-7) model, the IC30 value of WSP was lower than these of modified pectins, indicating decreased anti-Gal-3 activity. Our results suggested that the total amount of neutral sugar sidechains, the length of arabinan and cooperation between HG and RG-I played important roles in the anti-Gal-3 activity of WSP, not the Gal/Ara ratio or RG-I/HG ratio. These results provided a new insight into structure-activity relationship of citrus segment membrane RG-I as a galectin-3 antagonist and a new functional food.
Description
Carbohydrate Polymers, 245, 116526
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Structure-activity relationship of citrus segment membrane RG-I pectin against galectin-3: The galactan is not the only important factor, D. Wu, J. Zheng, W. Hu, X. Zheng, Q. He, R. J. Linhardt, X. Ye, S. Chen, Carbohydrate Polymers, 245, 116526, 2020.
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DOI
ISSN
1448617