High-Performance Liquid Chromatographic Separation of Heparin Derived Oligosaccharides
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https://orcid.org/0000-0003-2219-5833
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ENG
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Abstract
Heparin has been enzymatically depolymerized with heparinase (heparin lyase (EC 4.2.2.7)) and then separated into di-, tetra-, hexa-, octa-, and decasaccharide mixtures by low-pressure gel-permeation chromatography (GPC). These sized mixtures were resolved by strong anion-exchange (SAX) HPLC into multiple components. The fractions from the SAX-HPLC were collected and characterized for size by GPC-HPLC and sulfate content by ion chromatography. This study provides detailed methodology for the separation of larger and more highly sulfated oligosaccharides than previously reported. It describes the first use of ion chromatography for the accurate determination of the sulfate content of heparin oligosaccharides, a method which can also be applied to heparin and other glycosaminoglycans.
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Analytical Biochemistry, 150, 325-331
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
High-Performance Liquid Chromatographic Separation of Heparin Derived Oligosaccharides, K.G. Rice, Y.S. Kim, A.C. Grant, Z.M. Merchant, R.J. Linhardt, Analytical Biochemistry, 150, 325-331 (1985).
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10960309
32697
32697