High Resolution Crystal Structure of Arthrobacter aurescens Chondroitinase AC Complexed with Chondroitin Sulfate Defines the Catalytic Mechanism
No Thumbnail Available
Authors
Lunin, V.V.
Li, Y.
Linhardt, Robert J.
Miyazono, H.
Kyogashima, M.
Cygler, M.
Issue Date
2004
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate but not on dermatan sulfate. Like other chondroitin AC lyases, it is capable of cleaving hyaluronan.We have determined the three-dimensional crystal structure of ArthroAC in its native form as well as in complex with its substrates (chondroitin 4-sulfate tetrasaccharide, CStetra and hyaluronan tetrasaccharide) at resolution varying from 1.25 Å to 1.9 Å. The primary sequence of ArthroAC has not been previously determined but it was possible to determine the amino acid sequence of this enzyme from the high-resolution electron density maps and to confirm it by mass spectrometry. The enzyme–substrate complexes were obtained by soaking the substrate into the crystals for varying lengths of time (30 seconds to ten hours) and flash-cooling the crystals. The electron density map for crystals soaked in the substrate for as short as 30 seconds showed the substrate clearly and indicated that the ring of central glucuronic acid assumes a distorted boat conformation. This structure strongly supports the lytic mechanism where Tyr242 acts as a general base that abstracts the proton from the C5 position of glucuronic acid while Asn183 and His233 neutralize the charge on the glucuronate acidic group. Comparison of this structure with that of chondroitinase AC from Flavobacterium heparinum (FlavoAC) provides an explanation for the exolytic and endolytic mode of action of ArthroAC and FlavoAC, respectively.
Description
Journal of Molecular Biology, 337, 367-386
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
High Resolution Crystal Structure of Arthrobacter aurescens Chondroitinase AC Complexed with Chondroitin Sulfate Defines the Catalytic Mechanism, V. V. Lunin, Y. Li, R.J. Linhardt, H. Miyazono, M. Kyogashima, M. Cygler, Journal of Molecular Biology, 337, 367-386, 2004.