Capillary Electrophoresis of Oligosaccharides
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Authors
Linhardt, Robert J.
Issue Date
1994
Type
Book chapter
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
This chapter reports an overview of the recent advances in the analysis of mono- and oligosaccharides by capillary electrophoresis (CE); furthermore, relevant reviews and research articles recently published in the field are tabulated. Additionally, pretreatments and procedures applied to uncharged and acidic carbohydrates (i.e., monosaccharides and lower oligosaccharides carrying carboxylate, sulfate, or phosphate groups) are described.Representative examples of such procedures are reported in detail, upon describing robust methodologies for the study of (1) neutral oligosaccharides derivatized by reductive amination and by formation of glycosylamines; (2) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G; (3) anomeric couples of neutral glycosides separated using borate-based buffers; (4) unsaturated, underivatized oligosaccharides from lyase-treated alginate.
Description
in Methods in Enzymology, Guide to Techniques in Glycobiology, W.J. Lennarz, G.W. Hart (eds.), Volume 230, Chapter 16, pp 265-280, Academic Press, San Diego,
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Capillary Electrophoresis of Oligosaccharides R.J. Linhardt, in Methods in Enzymology, Guide to Techniques in Glycobiology, W.J. Lennarz, G.W. Hart (eds.), Volume 230, Chapter 16, pp 265-280, Academic Press, San Diego, 1994.