Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

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Authors
Zhi, Zijian
Chen, Jianle
Li, Shan
Wang, Wenjun
Huang, Rui
Liu, Donghong
DIng, Tian
Linhardt, Robert J. John
Chen, Shiguo
Ye, Xingqian
Issue Date
2017-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin faces great limitations as the large molecular size of pectin severely prevents its bioavailability in vivo. In this study, we report an effective and highly convenient approach to degrade natural pectin into lower molecular pectin. By combining ultrasound with Fenton system (US-Fenton), we show that ultrasound synergistically enhances the efficiency of Fenton reaction to degrade pectin into 5.5 kDa within only 35 minutes. Importantly, RG-I domain, the most effective portion of natural pectin, was well preserved and highly enriched. In addition, the antioxidant activities of US-Fenton-treated pectin was significantly elevated. The mechanism of this novel observation was further investigated through the multiple structural analyses including HPLC, IR and NMR. Taken together, we present a novel and convenient approach to generate ultra-low molecular weight pectin with high efficiency and higher bioactivity. We expect our approach will have broader applications in improving the bioavailability and bioactivity of other polysaccharide-based natural compounds.
Description
Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
Full Citation
Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process, Z. Zhi, J. Chen, S. Li, W. Wang, R. Huang, D. Liu, Tian Ding, R. J. Linhardt, S. Chen, X. Ye, Scientific Reports, 7, 541, 2017.
Publisher
Nature
Journal
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PubMed ID
DOI
ISSN
20452322
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