Addressing endotoxin issues in bioengineered heparin
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Authors
Suwan, Jiraporn
Torelli, Amanda
Onishi, Akihiro
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2012-11-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by bacterial fermentation and chemical and enzymatic processing is currently under development. This study examines the challenges of reducing or removing endotoxins associated with this process that are necessary to proceed with preclinical in vivo evaluation of bioengineered heparin. The current process is assessed for endotoxin levels, and strategies are examined for endotoxin removal from polysaccharides and enzymes involved in this process.
Description
Biotechnology and Applied Biochemistry, 59, 420-428
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Addressing endotoxin issues in bioengineered heparin, J. Suwan, A. Torelli, A. Onishi, J. S. Dordick, R. J. Linhardt, Biotechnology and Applied Biochemistry, 59, 420-428, 2012.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
14708744
8854513
8854513