NMR quantification for monitoring heparosan K5 capsular polysaccharide production
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Authors
Wang, Z.
Zhang, Z.
McCallum, Scott A.
Linhardt, Robert J.
Issue Date
2010
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive 1H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.
Description
Analytical Biochemistry, 398, 275–277
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
NMR quantification for monitoring heparosan K5 capsular polysaccharide production, Z. Wang, Z. Zhang, S. A. McCallum, R. J. Linhardt, Analytical Biochemistry, 398, 275–277, 2010.
Publisher
Elsevier