Influence of Formulation Methods on the In Vitro Controlled Release of Protein from Poly(Ester) Microspheres
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Authors
Wang, H.T.
Schmitt, E.
Flanagan, D.R.
Linhardt, Robert J.
Issue Date
1991
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Poly (dl-lactide/glycolide, 50:50) microspheres containing bovine serum albumin (BSA) were pre-pared with and without Carbopol® 951 (a potential adjuvant agent) by o/o, o/w and (w/o) /w emul-sion methods. The protein loading of the microspheres reached 50%–70% of the theoretical amount of protein put into the formulation medium. The microsphere particle size was approximately 500 μm, 25–100μm, 10–20/nn using o/o, o/w, or (w/o)/w emulsion techniques, respectively. The release of BSA was dependent on the preparation method. The greatest burst of release was found for vacuum-dried microspheres formulated using the (w/o)/w method. This burst effect could be eliminated by lyophilizing the microspheres following their preparation. BSA was released at a higher initial rate from microspheres prepared by the o/w emulsion method that contained Carbopol® 951 than from micro-spheres not containing Carbopol® 951. Release studies also suggested that the release of BSA could be sustained for 54, 36, or 34 days for microspheres prepared by o/o, o/w, or (w/o)/w methods, respectively.
Description
Journal of Controlled Release, 17, 23-32
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Influence of Formulation Methods on the In Vitro Controlled Release of Protein from Poly(Ester) Microspheres, H.T. Wang, E. Schmitt, D.R. Flanagan, R.J. Linhardt, Journal of Controlled Release, 17, 23-32 (1991).