Modified Assay for the Measurement of Biotin in the Presence of Heparin
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Authors
Nadkarni, V.D.
Linhardt, Robert J.
Issue Date
1995-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing buffer (Buffer 1) significantly decreased the chemiluminescence signals of both LMW-EGF and HMW-EGFs (data not shown). This result indicates that the binding of proteins to PVDF membranes is susceptible to the detergent even after fixation with formaldehyde.
Description
Analytical Biochemistry, 227, 402-404
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Modified Assay for the Measurement of Biotin in the Presence of Heparin, V. D. Nadkarni, R. J. Linhardt, Analytical Biochemistry, 227, 402-404, 1995.
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Terms of Use
Journal
Volume
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PubMed ID
DOI
ISSN
32697