Modified Assay for the Measurement of Biotin in the Presence of Heparin

Nadkarni, V.D.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Modified Assay for the Measurement of Biotin in the Presence of Heparin, V. D. Nadkarni, R. J. Linhardt, Analytical Biochemistry, 227, 402-404, 1995.
Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing buffer (Buffer 1) significantly decreased the chemiluminescence signals of both LMW-EGF and HMW-EGFs (data not shown). This result indicates that the binding of proteins to PVDF membranes is susceptible to the detergent even after fixation with formaldehyde.
Analytical Biochemistry, 227, 402-404
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Rensselaer Polytechnic Institute, Troy, NY
Analytical Biochemistry