Enabling scalable electrochemical cofactor regeneration via improved soluble expression and application of mutant renalase
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Authors
Morrison, Clifford Steven
Issue Date
2020-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical engineering
Alternative Title
Abstract
Therefore, devising engineering strategies for the improved soluble expression of hRen1 is necessary for enabling the recovery of adventitious reduced NAD(P)H cofactor material from electrochemical cofactor regeneration preparations. In this work, the primary sequence of hRen1 has been mutated and fused to various solubility proteins to enhance soluble expression of the enzyme, and their steady-state kinetics parameters are characterized relative to the wild type enzyme. Additionally, engineering strategies for utilizing electrochemical bioreactors for microbial electrosynthesis applications is explored.
Description
May 2020
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY