Production and characterization of recombinant collagen binding resilin nanocomposite for regenerative medicine applications

Mikael, Paiyz E.
Udangawa, Ranodhi
Sorci, Mirco
Cress, Brady
Shtein, Zvi
Belfort, Georges
Shoseyov, Oded
Dordick, Jonathan S.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Production and characterization of recombinant collagen binding resilin nanocomposite for regenerative medicine applications, P. E. Mikael, R. Udangawa, M. Sorci, B. Cress, Z. Shtein, G. Belfort, O. Shoseyov, J. S. Dordick, R. J. Linhardt, Regenerative Engineering and Translational Medicine, 5, 362–372, 2019.
Development of mechanically stable and multifunctional biomaterials for sensing, repair, and regeneration applications is of great importance. Herein, we investigate the potential of recombinant resilin-like (Res) nanocomposite elastomer as a template biomaterial for regenerative devices such as adhesive bandages or films, electrospun fibers, screws, sutures, and drug delivery vehicles. Exon I (Rec1) from the native resilin gene of Drosophila (CG15920) was fused with collagen-binding domain (ColBD) from Clostridium histolyticum and expressed in Komagataella pastoris (formerly Pichia pastoris). The 100% binding of Resilin-ColBD (Res-ColBD) to collagen I was shown at a 1:1 ratio by mass. Atomic force microscopy results in force mode show a bimodal profile for the ColBD-binding interactions. Moreover, based on the force-volume map, Res-ColBD adhesion to collagen was statistically significantly higher than resilin without ColBD.
Regenerative Engineering and Translational Medicine, 5, 362–372
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Regenerative Engineering and Translational Medicine