Production and characterization of recombinant collagen binding resilin nanocomposite for regenerative medicine applications
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Authors
Mikael, Paiyz E.
Udangawa, Ranodhi
Sorci, Mirco
Cress, Brady
Shtein, Zvi
Belfort, Georges
Shoseyov, Oded
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2019-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Development of mechanically stable and multifunctional biomaterials for sensing, repair, and regeneration applications is of great importance. Herein, we investigate the potential of recombinant resilin-like (Res) nanocomposite elastomer as a template biomaterial for regenerative devices such as adhesive bandages or films, electrospun fibers, screws, sutures, and drug delivery vehicles. Exon I (Rec1) from the native resilin gene of Drosophila (CG15920) was fused with collagen-binding domain (ColBD) from Clostridium histolyticum and expressed in Komagataella pastoris (formerly Pichia pastoris). The 100% binding of Resilin-ColBD (Res-ColBD) to collagen I was shown at a 1:1 ratio by mass. Atomic force microscopy results in force mode show a bimodal profile for the ColBD-binding interactions. Moreover, based on the force-volume map, Res-ColBD adhesion to collagen was statistically significantly higher than resilin without ColBD.
Description
Regenerative Engineering and Translational Medicine, 5, 362–372
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Production and characterization of recombinant collagen binding resilin nanocomposite for regenerative medicine applications, P. E. Mikael, R. Udangawa, M. Sorci, B. Cress, Z. Shtein, G. Belfort, O. Shoseyov, J. S. Dordick, R. J. Linhardt, Regenerative Engineering and Translational Medicine, 5, 362–372, 2019.
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Volume
Issue
PubMed ID
DOI
ISSN
23644141
23644133
23644133