Chemobiocatalytic Synthesis of a Low Molecular Weight Heparin
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Authors
Yu, Yanlei
Fu, Li
He, Peng
Xia, Ke
Varghese, Sony
Wang, Hong
Zhang, Fuming
Dordick, Jonathan
Linhardt, Robert J.
Issue Date
2022-03-18
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin products are widely used clinical anticoagulants essential in the practice of modern medicine. Low-molecular-weight heparins (LMWHs) are currently prepared by the controlled chemical or enzymatic depolymerization of unfractionated heparins (UFHs) that are extracted from animal tissues. In many clinical applications, LMWHs have displaced UFHs and currently comprise over 60% of the heparin market. In the past, our laboratory has made extensive efforts to prepare bioengineered UFHs relying on a chemoenzymatic process to address concerns about animal-sourced UFHs. The current study describes the use of a novel chemoenzymatic process to prepare a chemobiosynthetic LMWH from a low-molecular-weight heparosan. The resulting chemobiocatalytic LMWH matches most of the United States pharmacopeial specifications for enoxaparin, a LMWH prepared through the base-catalyzed depolymerization of animal-derived UFH.
Description
ACS Chemical Biology,17, 637-646
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Chemobiocatalytic Synthesis of a Low Molecular Weight Heparin, Y. Yu, L. Fu, P. He, K. Xia, S. Varghese, H. Wang, F. Zhang, J. Dordick, R.J. Linhardt, ACS Chemical Biology,17, 637-646, 2022.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15548937
15548929
15548929