Development of bioactive biomaterials for pluripotent stem cell arterial venous differentiation

Authors
Dorsey, Taylor Braam
ORCID
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Other Contributors
Dai, Guohao
Thompson, Deanna M.
Hahn, Mariah
Vincent, Peter
Issue Date
2016-12
Keywords
Biomedical engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
Within the framework of this thesis, we proposed to investigate ephrinB2/EphB4 signaling with respect to vascular and arterial venous pluripotent stem cell fate in vitro, specifically utilizing biomaterial approaches to mimic cell-cell signaling. PEG hydrogel photochemistry was optimized to create bioactive platforms with the potential to spatially-control the immobilization proteins or peptides. We successfully created distinct arterial venous EC populations derived from pluripotent stem cells using a combination of immobilized and soluble biochemical cues and thoroughly characterized these populations for phenotypic functionality. EphrinB2 signaling mechanisms during differentiation in vitro and furthermore how multivalency plays a role was explored. Overall, the findings of this thesis will help further our understandings of arterial venous specification, highlight the importance of phenotypic-specific ECs and provide novel biomaterial approaches to control stem cell fate.
Description
December 2016
School of Engineering
Department
Dept. of Biomedical Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
Access
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