Bacteriophage T7 transcription system: an enabling tool in synthetic biology

Wang, Wenya
Li, Yuwenbin
Wang, Yaqiong
Shi, Chen
Li, Chenmeng
Li, Qiang
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Bacteriophage T7 transcription system: an enabling tool in synthetic biology, W. Wang, Y. Li, Y. Wang, C. Shi, C. Li, Q. Li, R. J. Linhardt, Biotechnology Advances, 36, 2129–2137, 2018.
Since its discovery in the 1970s, the T7 RNA polymerase (T7 RNAP) transcription system has been applied extensively as an effective tool in molecular biology because of its robust function in various hosts, including prokaryotic, eukaryotic and cell free systems. Recently, the T7 RNAP transcription system has emerged as a critical component for synthetic biology. The present paper summarizes the advances of the T7 RNAP transcription system in synthetic biology, including the recent progress of T7 RNAP structure and its cognate promoter and terminator and its application in cell free systems, logic gates and orthogonal genetic circuits.
Biotechnology Advances, 36, 2129–2137
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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Rensselaer Polytechnic Institute, Troy, NY
Biotechnology Advances