Selective, switchable fluorescent probe for heparin based on aggregation-induced emission
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Authors
Wang, Yi Jia
Lin, Lei
Zhang, Xing
Schultz, Victor
Zhang, Fuming
Sun, Jing Zhi
Linhardt, Robert J.
Issue Date
2016-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A positively charged tetraphenylethene (TPE) derivative, TPE-4MN, was synthesized as a probe for heparin based on aggregation induced emission. On the addition of 5.0 μg/mL of heparin, TPE-4MN showed an enhanced emission of about 10-fold. The change in fluorescence at 475 nm was linear over a range of heparin concentrations of 0–1.0 μg/mL with an R = 0.99988 and the limit of detection (LOD) was calculated to be 0.75 μg/mL. The mechanism of the detection was proven to be through an ion pairing interaction. TPE-4MN showed good selectivity for heparin over other types of polysaccharides and could easily distinguish heparin from heparan sulfate, a glycosaminoglycan having a similar structure to that of heparin.
Description
Analytical Biochemistry, 514, 48-54
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Selective, switchable fluorescent probe for heparin based on aggregation-induced emission, Y.-J. Wang, L. Lin, X. Zhang, V. Schultz, F. Zhang, J.- Z. Sun, R. J. Linhardt, Analytical Biochemistry, 514, 48-54, 2016.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697