Preparation of low molecular weight heparins through nitrous acid degradation from bovine and ovine heparins

Xie, Shaoshuai
Guan, Yudong
Zhu, Peipei
Li, Fei
Yu, Mengqi
Linhardt, Robert J.
Chi, Lianli
Jin, Lan
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Preparation of low molecular weight heparins through nitrous acid degradation from bovine and ovine heparins, S. Xie, Y. Guan, P. Zhu, F. Li, M. Yu, R. J. Linhardt, L. Chi, L. Jin, Carbohydrate Polymers, 197, 83-91, 2018.
Low molecular weight heparins (LMWHs) are important anticoagulant drugs. Nitrous acid degradation is a major approach to produce LMWHs, such as dalteparin. Due to the foreseeable shortage of porcine intestinal mucosa heparin and other potential risks, expansion of other animal tissues for heparin preparation is necessary. Heparins from different tissues differ in structure and bioactivity potency, and these variations may be carried over to the LMWH products. Sophisticated analytical techniques have been applied to compare various versions of dalteparins produced from porcine intestinal, bovine lung and ovine intestinal heparins to elucidate the effects of different animal tissues starting materials and processing conditions on the properties of final dalteparin products. With adjusted depolymerization conditions, versions of dalteparins that qualify under the European Pharmacopeia (EP) specifications were manufactured using non-porcine heparins. Dissimilarities among the three interspecies animal tissue heparin-derived dalteparins regarding fine structures are also disclosed, and their origins are discussed.
Carbohydrate Polymers, 197, 83-91
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Polymers