Characterization of the thymidine kinase in herpes virus-transformed cells

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Authors
Staczek, John
Issue Date
1976-12
Type
Electronic thesis
Thesis
Language
ENG
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Biology
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Abstract
Herpes simplex virus Type 2 (HSV-2) also codes for a thymidine kinase which is different from the cytosol or mitochondrial forms of the enzyme. Cells lytically infected with HSV-2 have a viral enzyme with a PAGE mobility intermediate between the cytosol and mitochondrial thymidine kinases. The viral enzyme used CTP as a phosphate donor and was very heat sensitive. Two HSV-2 transformed cell lines showed no evidence of any viral thymidine kinase when tested for their CTP specificity or thermostability. PAGE profiles showed no detectable viral enzyme activity.
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December 1976
School of Science
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Rensselaer Polytechnic Institute, Troy, NY
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