Engineering biomolecular interactions to enhance selectivity in chromatographic separations

Authors
Woo, James
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Other Contributors
Cramer, Steven M.
Karande, Pankaj
Breneman, Curt M.
Tessier, Peter M.
Linhardt, Robert J.
Issue Date
2014-12
Keywords
Chemical and biological engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
Studies of increasingly complex peptide-based ligands showed that the proper presentation of complementary chemical properties to a targeted protein surface could be used to design ligands with enhanced affinity and selectivity for a target protein. Using a combination of high-throughput techniques consisting of microarray screening and 96-well plate experiments, a high-affinity peptide ligand was selected from the initial library (designed in silico) and rapidly scaled up to a small-scale column that could separate a target protein from harvested cell culture fluid.
Description
December 2014
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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