Engineering biomolecular interactions to enhance selectivity in chromatographic separations

Abstract

Studies of increasingly complex peptide-based ligands showed that the proper presentation of complementary chemical properties to a targeted protein surface could be used to design ligands with enhanced affinity and selectivity for a target protein. Using a combination of high-throughput techniques consisting of microarray screening and 96-well plate experiments, a high-affinity peptide ligand was selected from the initial library (designed in silico) and rapidly scaled up to a small-scale column that could separate a target protein from harvested cell culture fluid.