Engineering biomolecular interactions to enhance selectivity in chromatographic separations
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Authors
Woo, James
Issue Date
2014-12
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical and biological engineering
Alternative Title
Abstract
Studies of increasingly complex peptide-based ligands showed that the proper presentation of complementary chemical properties to a targeted protein surface could be used to design ligands with enhanced affinity and selectivity for a target protein. Using a combination of high-throughput techniques consisting of microarray screening and 96-well plate experiments, a high-affinity peptide ligand was selected from the initial library (designed in silico) and rapidly scaled up to a small-scale column that could separate a target protein from harvested cell culture fluid.
Description
December 2014
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY