Structural and activity variability of fractions with different charge density and chain length from pharmaceutical heparins
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Authors
Yu, Yanlei
Hirakane, Makoto
Mori, Daisuke
Lin, Lei
Zhang, Fuming
Zhang, Hong
Linhardt, Robert J.
Issue Date
2017-08-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin is a structurally complex polysaccharide used as a clinical anticoagulant. It is comprised of a heterogeneous mixture of polysaccharide chains having a variety of sequences and lengths. The production methods and regulatory controls of pharmaceutical heparins have changed over the years. This study assesses the structural and activity uniformity of the polysaccharide chains comprising two contemporary heparin products. The heparin fractions with different sizes and charges were separated with size exclusion and ion exchange chromatography. The fractions were analyzed for their molecular weight properties, di- and tetrasaccharide compositions, and anti-factor IIa and anti-factor-Xa activities. The distribution of these properties through chains of different lengths and ones with different charge density were compared. The results demonstrate that with the increase in heparin purity, activity and molecular weight required by the current pharmacopeia, the uniformity of pharmaceutical heparin products have increased.
Description
Glycoconjugate Journal, 34, 545–552
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Structural and activity variability of fractions with different charge density and chain length from pharmaceutical heparins, Y. Yu, M. Hirakane, D. Mori, L. Lin, F. Zhang, H. Zhang, R. J. Linhardt, Glycoconjugate Journal, 34, 545–552, 2017.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15734986
2820080
2820080