Antithrombin III Binding Site Analysis of Low Molecular Weight Heparin Fractions

Chen, Yin
Zhao, Jing
Yu, Yanlei
Liu, Xinyue
Lin, Lei
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Antithrombin III Binding Site Analysis of Low Molecular Weight Heparin Fractions, Y Chen, J Zhao, Y Yu, X Liu, L Lin, F Zhang, R. J. Linhardt, Journal of Pharmaceutical Sciences, 107, 1290-1295, 2018.
Low-molecular-weight heparins (LMWHs) are widely used as clinical anticoagulant drugs. LMWHs are heterogeneous and highly negatively charged glycans prepared by chemical or enzymatic depolymerization of unfractionated heparin. The detailed structural analysis of a LMWH is essential for the drug quality control. In this study, an LMWH, enoxaparin sodium (a generic version of Lovenox) was separated into different molecular weight fractions by a Superdex peptide column. The disaccharide compositions, 3-O-sulfo group-containing tetrasaccharides composition, and antithrombin III-binding affinity of the fractions from this LMWH were analyzed. The results showed that all the fractions had very similar disaccharide and 3-O-sulfo group-containing tetrasaccharide compositions, but the fraction containing larger-sized chains had higher antithrombin III-binding affinity.
Journal of Pharmaceutical Sciences, 107, 1290-1295
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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Rensselaer Polytechnic Institute, Troy, NY
Journal of Pharmaceutical Sciences