Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC

Authors
Toida, T.
Shima, M.
Azumaya, S.
Maruyama, T.
Toyoda, H.
Imanari, T.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1997
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC,T. Toida, M. Shima, S. Azumaya, T. Maruyama, H. Toyoda, T. Imanari, R.J.Linhardt, Journal of Chromatography, 787, 266-270, 1997.
Abstract
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
Description
Journal of Chromatography, 787, 266-270
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
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