Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC

Toida, T.
Shima, M.
Azumaya, S.
Maruyama, T.
Toyoda, H.
Imanari, T.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC,T. Toida, M. Shima, S. Azumaya, T. Maruyama, H. Toyoda, T. Imanari, R.J.Linhardt, Journal of Chromatography, 787, 266-270, 1997.
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
Journal of Chromatography, 787, 266-270
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