Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC
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Authors
Toida, T.
Shima, M.
Azumaya, S.
Maruyama, T.
Toyoda, H.
Imanari, T.
Linhardt, Robert J.
Issue Date
1997
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
Description
Journal of Chromatography, 787, 266-270
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Detection of Glycosaminoglycans as a Copper (II) Complex in HPLC,T. Toida, M. Shima, S. Azumaya, T. Maruyama, H. Toyoda, T. Imanari, R.J.Linhardt, Journal of Chromatography, 787, 266-270, 1997.