Combined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Authors
Liu, Tengfei
Li, Jun
Qiu, Lequan
Zhang, Fuming
Linhardt, Robert J.
Zhong, Weihong
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2020-12-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Full Citation
Combined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW, T. Liu, J. Li, L. Qiu, F. Zhang, R. J. Linhardt, W. Zhong, Biotechnology and Bioengineering, 117, 3712–3726. 2020.
Abstract
Dibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of a river of Hangzhou, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP compared with glucose revealed specific DBP metabolic pathways in the ZJUTW strain. The degrading gene clusters localize separately on a circular chromosome and a plasmid pQL1. Genes related to the initial steps of DBP degradation from DBP to phthalic acid (PA), the pehA gene, and pht gene cluster, are located on the plasmid pQL1. However, the pca gene cluster related to the transforming of intermediate protocatechuic acid (PCA) to acetyl-CoA, is located on the chromosome. After comparative analysis with the reported gene clusters, we found that there were a series of homologous genes in pht and pca gene clusters that contribute to the efficient degradation of DBP by ZJUTW. In addition, transcriptomic analysis suggested a synergistic effect between pht and pca clusters, which also favor ZJUTW allowing it to efficiently degrade DBP. Combined genomic and transcriptomic analyses revealed a complete DBP metabolic pathway in Arthrobacter sp. ZJUTW that is different from that of other reported Arthrobacter strains. After necessary modification based on its metabolic characteristics, Arthrobacter sp. ZJUTW or its derivatives might represent promising candidates for the bioremediation of DBP pollution.
Description
Biotechnology and Bioengineering, 117, 3712–3726
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Biotechnology and Bioengineering
https://harc.rpi.edu/
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