Capillary Electrophoresis for the Analysis of Glycosaminoglycans and Glycosaminoglycan-Derived Oligosaccharides
Loading...
Authors
Mao, W.J.
Thanawiroon, C.
Linhardt, Robert J.
Issue Date
2002-03-20
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.
Description
Biomedical Chromatography, 16, 77-94
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Capillary Electrophoresis for the Analysis of Glycosaminoglycans and Glycosaminoglycan-Derived Oligosaccharides, W.J. Mao, C. Thanawiroon, R.J. Linhardt, Biomedical Chromatography, 16, 77-94, 2002.
Publisher
Wiley
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
2693879