Preparation and application of a “clickable” acceptor for enzymatic synthesis of heparin oligosaccharides
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Authors
Cai, Chao
Edgar, Kristi
Liu, Jian
Linhardt, Robert J.
Issue Date
2013-05-03
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A 'clickable' disaccharide was prepared by treating the aldehyde precursor with hydroxylamine, followed by the catalytic hydrogenation and diazotransfer reaction. This disaccharide was successfully applied to the elongation of the backbone construction of ultralow molecular weight (ULMW) heparins using two bacterial glycosyl transferases, N-acetyl glucosaminyl transferase from Escherichia coli K5 (KfiA) and heparosan synthase-2 (pmHS2) from Pasteurella multocida.
Description
Carbohydrate Research, 372, 30–34
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Preparation and application of a “clickable acceptor for enzymatic synthesis of heparin oligosaccharides," C. Cai, K. Edgar, J. Liu, R.J. Linhardt, Carbohydrate Research, 372, 30–34, 2013.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
1873426X
86215
86215