A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide

Yan, L.
Fu, L.
Xia, K.
Chen, S.
Zhang, F.
Dordick, J.S.
Linhardt, Robert J.
Thumbnail Image
Other Contributors
Issue Date
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide, L. Yan, L. Fu, K. Xia, S. Chen, F. Zhang, J. S. Dordick, R.J. Linhardt, Biomolecules, 10, 1516, 2020.
The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the glycolipid acceptor structure, from which heparosan is extended, have been corrected. The structure of heparosan CPS is GlcNAc α-1,[4GlcA β-1,4GlcNAc α-1,]n4GlcA β-1,[4Kdo β-2,7Kdo β-2,]0 or 14Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-PG-I (C16:0 or C18:0) (where n is ~250 for a CPS of 100 kDa).
Biomolecules, 10, 1516
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Multidisciplinary Digital Publishing Institute (MDPI)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Open Access
CC BY — Creative Commons Attribution