A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide
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Authors
Yan, L.
Fu, L.
Xia, K.
Chen, S.
Zhang, F.
Dordick, J.S.
Linhardt, Robert J.
Issue Date
2020
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the glycolipid acceptor structure, from which heparosan is extended, have been corrected. The structure of heparosan CPS is GlcNAc α-1,[4GlcA β-1,4GlcNAc α-1,]n4GlcA β-1,[4Kdo β-2,7Kdo β-2,]0 or 14Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-PG-I (C16:0 or C18:0) (where n is ~250 for a CPS of 100 kDa).
Description
Biomolecules, 10, 1516
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide, L. Yan, L. Fu, K. Xia, S. Chen, F. Zhang, J. S. Dordick, R.J. Linhardt, Biomolecules, 10, 1516, 2020.
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)