Glycocalyx-like hydrogel coatings for small diameter vascular grafts
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Authors
Dimitrievska, Sashka
Wang, Juan
Lin, Tylee
Weyers, Amanda
Bai, Hualong
Qin, Lingfeng
Li, Guangxin
Cai, Chao
Kypson, Alan
Kristofik, Nina
Issue Date
2020-06-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Novel biological vascular conduits, such as decellularized tissue engineered vascular grafts (TEVGs) are hindered by high thrombogenicity. To mimic the antithrombogenic surface of native vessels with a continuous glycosaminoglycan layer that is present on endothelial cells (ECs), a hyaluronic acid (HA) modified surface is established, to effectively shield blood platelets from collagen-triggered activation. Using the amine groups present on 4 mm diameter decellularized TEVGs, a continuous HA hydrogel coating is built via a bifunctional thiol-reactive cross-linker, thereby avoiding nonspecific collagen matrix cross-linking. The HA hydrogel layer recreates a luminal wall, “hiding” exposed collagen from the bloodstream. In vitro blood tests show that adhered platelets, fibrinogen absorption, and fibrin formation on HA-coated decellularized TEVGs are significantly lower than on uncoated decellularized TEVGs. The HA surface also inhibits macrophage adhesion in vitro. HA-coated decellularized syngeneic rat aortae (≈1.5 mm diameter), and TEVGs in rat and canine models, respectively, are protected from aggressive thrombus formation, and preserve normal blood flow. Re-endothelialization is also observed. HA-coated TEVGs may be an off-the-shelf small-diameter vascular graft with dual benefits: antithrombogenic protection and promotion of endothelium.
Description
Advanced Functional Matererials, 30, 1908963
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Glycocalyx-like hydrogel coatings for small diameter vascular grafts, S. Dimitrievska, J. Wang, T. Lin, A. Weyers, H. Bai, L. Qin, G. Li, C. Cai, A. Kypson, N. Kristofik, A. Gard, S. Sundaram, K. Yamamoto, W. Wu, L. Zhao, M. H. Kural, Y. Yuan, J. Madri, T. R. Kyriakides, R. J. Linhardt, L. E. Niklason, Advanced Functional Matererials, 30, 1908963, 2020.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
16163028
1616301X
1616301X